Fig. 4: Impact of Hb overexpression on the accumulation of C-terminal truncated α-syn. | Cell Death & Disease

Fig. 4: Impact of Hb overexpression on the accumulation of C-terminal truncated α-syn.

From: Neuronal haemoglobin induces loss of dopaminergic neurons in mouse Substantia nigra, cognitive deficits and cleavage of endogenous α-synuclein

Fig. 4

Representative Western blot of lysates from Hb and CTRL cells upon supplementation of vehicle, Ms and PFFs for 24 h (a). Representative confocal microscopy images of Hb cells supplemented with Alexa-488 labelled PFFs for 24 h. Entire cells were labelled by CellMask. Untransfected cells incubated only with secondary antibody were used to establish autofluorescence levels. Nuclei were stained with DAPI. Scale bar 10 μm (b). CTRL and Hb cells were treated with α-syn amyloids. Cell lysates were collected at the indicated time points and analyzed by immunoblotting with SYN-1 (c) and C-20 antibodies (d). Band intensity corresponding to ΔC-α-syn and FL-α-syn was quantified and the ratio was calculated. Data represent means ± SEM and are representative of six independent experiments. Statistical analysis was performed with one-way Anova. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001 (e).

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