Fig. 5: Effect of Calpain I inhibition on α-syn C-terminal truncated species accumulation in Hb cells. | Cell Death & Disease

Fig. 5: Effect of Calpain I inhibition on α-syn C-terminal truncated species accumulation in Hb cells.

From: Neuronal haemoglobin induces loss of dopaminergic neurons in mouse Substantia nigra, cognitive deficits and cleavage of endogenous α-synuclein

Fig. 5

Cell lysates of Hb cells treated with DMSO (-) and Calpain inhibitor III (+) were analyzed by immunoblotting with SYN-1 (a) and C-20 (b) antibodies. Band intensity corresponding to ΔC-α-syn and FL-α-syn was quantified and the ratio was calculated. Data represent means ± SEM and are representative of six independent experiments. Statistical analysis was performed with one-way Anova. *p ≤ 0.05; **p ≤ 0.01 (c). Cell lysates of Hb cells treated with DMSO (-) and CI-III (+) were analyzed by immunoblotting with anti-Spectrin α II antibody (d).

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