Fig. 3: XPA knockout combats the abiraterone resistance.
A, B Abiraterone-resistant cells (LNCaP/ABI and 22RV1/ABI) and their parental control cells were treated with DMSO, ABI (10 μM), IDA (0.25 μM), and combination ABI with IDA. XPA expressions were determined by western blot. C XPA expression of tumor tissues from mice administered as shown in Fig. 2A was determined by IPC. Scale bar, 200 μm. D LNCaP/ABI and 22RV1/ABI cells were treated with DMSO, ABI (10 μM), IDA (0.25 μM), and combination ABI with IDA. Expressions of γH2AX and AR were determined by western blot. E–G LNCaP/ABI cells (1 × 106) were infected with lentivirus expressing sgSCR and sgXPA and then injected subcutaneously into the flank of 8-week-old male castrated mull mice. When tumor size reached about 50 mm3, mice were administered with vehicle (10% DMSO plus 90% corn oil mixture as control, n = 8), AA (0.5 mmol/kg, i.g.), (n = 10, per group) (E). Tumor volume (F) and survival (G) were monitored as indicated days. Data are presented as means ± s.e.m. Statistical analysis for tumor growth and overall animal survival were performed using two-way ANOVA analysis (F) and Log-rank (Mantel-Cox) test analysis (G), respectively. The asterisks indicate significant differences between the indicated groups (*p < 0.05, ***p < 0.001).
