Fig. 3: A LOI mutant HP mimetic is more toxic through RNAi than the reference HP. | Cell Death & Disease

Fig. 3: A LOI mutant HP mimetic is more toxic through RNAi than the reference HP.

From: The length of uninterrupted CAG repeats in stem regions of repeat disease associated hairpins determines the amount of short CAG oligonucleotides that are toxic to cells through RNA interference

Fig. 3

A Secondary structures of the synthesized RNA HPs including two control HPs comprised of pure CAG repeats. Different TNRs are shown in different colors. Binding energies are given. B, left, change in confluency over time of NB7 cells transfected with three of the HPs at 2.5 nM. B, right, viability (ATP assay) of NB7 cells 4 days after transfection with the different HPs (1 nM). Fold change is shown relative to lipid treated cells. Two-way ANOVA is shown. C Change in confluency over time of HeLa parental or AGO2 k.o. cells transfected with four of the HPs at 25 nM. D, left panel, viability of HeLa parental or AGO2 k.o. cells 4 days after transfection with the different HPs (25 nM). D, right panel, E Viability of indicated cells 4 days after transfection with the different HPs (10 nM for 293T cells, 2.5 nM for HCT116 cells). Fold change is shown relative to (CAG)29 control. Average of 3–6 replicates are shown −/+SE (B, left panel, C) and mean of 3-6 replicates are shown −/+SD (B, right panel, D, E). par = parental. P-values (Student’s t-test) are shown in B, right panel, D and E. ns, not significant. Inserts show Western blot analyses of the parental and corresponding mutant cell lines.

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