Fig. 2: A distal super-enhancer activates ETS2 transcription through long-range interaction in CRC cell lines. | Cell Death & Disease

Fig. 2: A distal super-enhancer activates ETS2 transcription through long-range interaction in CRC cell lines.

From: A distal super-enhancer activates oncogenic ETS2 via recruiting MECOM in inflammatory bowel disease and colorectal cancer

Fig. 2

A Expression level of ETS2 in CRC cell lines and a normal colonic epithelial cell line CCD-18Co as determined by RT-qPCR. SE, super-enhancer. B IGV tracks showing the H3K27ac ChIP-seq profiles of indicated samples across ETS2 promoter and its potential distal SE. Bridging curves indicate the interactions between the distal SE and ETS2 promoter detected by pc-HiC experiments in indicated cell lines. The sites targeted by guide RNAs that recruits dCas9-KRAB were indicated at the bottom. C Left panel: PCR results for 3C samples or control samples using primers targeting indicated regions (fragments in ETS2 promoter or its distal SE). A pair of primers that amplifying a region nearby ETS2 promoter that does not contain any EcoRI restriction site inside were used as positive controls. Right panel: Sanger sequencing results of indicated PCR products from 3C experiments. D Upper panel: diagram showing the principle of the dCas9-KRAB targeted transcription repression system. Bottom panel: expression level of ETS2 in cells expressing sgRNAs targeting indicated enhancer fragments or a control intergenic region together with the dCas9-KRAB fusion protein. **p < 0.01; n.s. non-significant.

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