Fig. 4: Oncogenic transcription factor MECOM is recruited to the ETS2-SE for ETS2 transcription activation. | Cell Death & Disease

Fig. 4: Oncogenic transcription factor MECOM is recruited to the ETS2-SE for ETS2 transcription activation.

From: A distal super-enhancer activates oncogenic ETS2 via recruiting MECOM in inflammatory bowel disease and colorectal cancer

Fig. 4

A Result of motifbreak analysis of the IBD-risk SNP (rs2836754) in ETS2-SE showing preferential binding of MECOM/EVI1 at T allele over C allele. B IGV tracks showing the MECOM and H3K27ac ChIP-seq profiles across indicated region in Lovo cells. C Enrichment of MECOM binding at the IBD-risk SNP (rs2836754) site in ETS2-SE as determined by CUT&Tag followed by qPCR analysis. *p < 0.05; **p < 0.01. D Expression of MECOM and ETS2 in CRC cells stably expressing shRNAs targeting MECOM or a non-targeting shRNA were examined by RT-qPCR. *p < 0.05; **p < 0.01. E Scatter plots showing the correlation between MECOM and ETS2 levels in CRC and IBD samples. PCC Pearson Correlation Coefficient. F Western blot results showing the expression of MECOM and ETS2 in CRC cell lines and a normal colonic epithelial cell line CCD-18Co. GAPDH was used as a loading control. Bands representing different isoforms of MECOM/EVI1 protein were indicated by black arrows.

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