Fig. 4: P300 mediates DLD 2-hydroxyisobutyrylation. | Cell Death & Disease

Fig. 4: P300 mediates DLD 2-hydroxyisobutyrylation.

From: Rg3 regulates myocardial pyruvate metabolism via P300-mediated dihydrolipoamide dehydrogenase 2-hydroxyisobutyrylation in TAC-induced cardiac hypertrophy

Fig. 4

A HEK293 cells, with or without C646, were transfected with indicated plasmid and harvested for immunoprecipitation and immunoblot analysis with the indicated antibodies. The representative immnoublots were presented, n = 3 for each group. B, C Heart homogenates of the Sham and TAC group were immunoprecipitated with anti-DLD or anti-2-hydroxyisobutyryl-lysine agarose beads, and probed with anti-2-hydroxyisobutyryl-lysine, anti-DLD, and anti-β-actin antibody. The representative immnoublots were presented, n = 3 for each group. D, E Representative digital western blots of P300, HDAC2, and HDAC3, and corresponding quantitative data, n = 3 for each group. F The reciprocal immunoprecipitaiton experiment showed that DLD and P300 were complexed in ACMs, n = 3 for each group. G DLD and P300 constituted a complex in the HEK293 cells, n = 3 for each group. The data were analyzed using Kruskal Wallis test and were expressed as means ± SD, *p < 0.05.

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