Fig. 6: Rg3 Downregulated DLD 2-Hydroxyisobutyrylation and Regulated Pyruvate Metabolism by P300. | Cell Death & Disease

Fig. 6: Rg3 Downregulated DLD 2-Hydroxyisobutyrylation and Regulated Pyruvate Metabolism by P300.

From: Rg3 regulates myocardial pyruvate metabolism via P300-mediated dihydrolipoamide dehydrogenase 2-hydroxyisobutyrylation in TAC-induced cardiac hypertrophy

Fig. 6

A, B Representative digital western blots for P300 and β-actin, and corresponding quantitative data, n = 3 for each group. C Molecular docking showing Rg3 in the binding site of P300. D Representative digital western blots for P300 and β-actin, n = 3 for each group. E Schedule of animal treatments. AVV9-GV469 and AVV9-GV469-P300 were injected intracardiacally. TAC operation was performed four weeks later. Rg3 or vehicle was administered to TAC mice the fourth week after surgery and maintained by one administration per day for 4 weeks. F Heart homogenates were immunoprecipitated with anti-DLD agarose beads, and probed with anti-2-hydroxyisobutyryl-lysine, anti-DLD, and anti-β-actin antibody. The representative immnoublots were presented, n = 3 for each group. G, H The contents of pyruvate and acetyl-CoA in heart tissue, n = 3 for each group. I The activity of pyruvate dehydrogenase in heart tissue, n = 3 for each group. J Representative images showing gross cardiac morphology of mice in each group. HE staining of longitudinal heart. WGA staining, n = 5–8 for each group. The data were analyzed using Kruskal–Wallis test and were expressed as means ± SD, #p < 0.05, ##p < 0.01 versus Sham group, *p < 0.05, **p < 0.01 versus TAC group.

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