Fig. 1: Wwox deletion synergies with KrasG12D activation by accelerating neoplastic lesions formation. | Cell Death & Disease

Fig. 1: Wwox deletion synergies with KrasG12D activation by accelerating neoplastic lesions formation.

From: Loss of tumor suppressor WWOX accelerates pancreatic cancer development through promotion of TGFβ/BMP2 signaling

Fig. 1

A Illustration of KWC mice generation, Ptf1a-CreER mice were crossed with Rosa26-LSL- tdTomato to generate WT mice. WT mice were crossed with Kras + /LSL-G12D mice to generate KC mice. KC mice were crossed with (Wwoxf/f) mice to generate either KWC or WC mice. B Immunofluorescence of tdTomato (magnification, ×40), immunostaining of WWOX and pERK respectively in KWC, and WT mice (magnification, ×20). C Representative haematoxylin and eosin (H&E) stain of KC and KWC pancreata at 1, 2, 3, 4, 5, and 6 months, respectively, (magnification, ×4), n ≥ 3 for each time point. D Quantification of ADM and PanIN lesions in KC and KWC mice in 4-, 8-, and 12-week post-tamoxifen injection. Number of KC mice, n = 4 at 1 month, n = 4 at 2 months, and n = 3 at 3 months. Number of KWC mice, n = 9 at 1 month, n = 4 at 2 months, and n = 3 at 3 months. E Representative Alcian blue stain of KC and KWC mice, 1-month post-tamoxifen injection (magnification, ×40). F Representative Ck-19 staining of KC (n = 4) and KWC (n = 9) mice, 1-month post-tamoxifen injection (magnification, ×20). G Quantification of Ck-19 in F. H Representative Ki-67 staining of pancreata of KC (n = 4) and KWC (n = 9) mice, 1-month post-tamoxifen injection (magnification, ×20). I Quantification of Ki-67 in H. NS > 0.05, *P < 0.05, **P < 0. 001, ***P < 0. 0001. Two-tail unpaired t-test. Quantification graphs are presenting the means ± SD.

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