Fig. 4: Wwox deletion promotes TGFβ and BMP2 through SMADs signaling. | Cell Death & Disease

Fig. 4: Wwox deletion promotes TGFβ and BMP2 through SMADs signaling.

From: Loss of tumor suppressor WWOX accelerates pancreatic cancer development through promotion of TGFβ/BMP2 signaling

Fig. 4

A Immunoblotting of pSMAD 2/3, pSMAD 1/5/9, and BMP2 of WT (n = 2), KC (n = 3), KWC Wwoxf/+ (n = 3), and KWC Wwoxf/f (n = 2) mice 1-month post-tamoxifen injection. B, C RT-PCR of Serpine1 and Angptl4 of WT, KC, and KWC mice 1-month post-tamoxifen injection. D RT-PCR of Runx2 of WT, KC, and KWC mice 1-month post-tamoxifen injection. E Representative immunofluorescence staining of pERK of KC, and KWC mice 1-month post-tamoxifen injection (magnification, ×60). F Quantification of pERK staining of KC (n = 4) and KWC (n = 5). G Immunoprecipitation of endogenous WWOX from 266-6 cells not activated or activated (+dox) for 24 h. Whole-cell lysates were immunoprecipitated with either rabbit IgG (control) or monoclonal WWOX mouse antibodies. The immunoprecipitants were immunoblotted with anti-WWOX, anti-pSMAD 2/3, anti-pERK, anti-ERK, and anti-BMP2/4 as indicated. **P < 0. 001, ***P < 0. 0001. Two-tail unpaired t-test. Quantification graphs are presenting the means ± SD.

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