Fig. 5: WWOX ablation accelerates PDAC formation in mice. | Cell Death & Disease

Fig. 5: WWOX ablation accelerates PDAC formation in mice.

From: Loss of tumor suppressor WWOX accelerates pancreatic cancer development through promotion of TGFβ/BMP2 signaling

Fig. 5

A 16 out of 64 Wwoxf/+ or Wwoxf/f KWC mice formed tumors between the ages 4–8 months while none of the KC mice (n = 14) did in this time range. B. Left panel—representative H&E stain of normal and carcinoma site of KWC mice (magnification, ×10). Right panel—representative immunofluorescence stain of tdTomato of normal and carcinoma site of KWC mice (magnification, ×40). C Representative WWOX, Pancytokeratin, pERK, pStat3, and Amylase immunochemical stain of KWC mice at normal and carcinoma sites (magnification, ×10). D Representative immunofluorescence stain of tdTomato, E-cadherin, and Vimentin of KWC mice at carcinoma site (magnification, ×40). E Representative IHC staining of BMP2 at tumor site of a mouse at age of 8 months, and at spleen site of the same mouse (magnification, ×40). F Upper panel- H&E stain of liver, lung, and spleen (magnification, ×10). Representative immunofluorescence stain of tdTomato of metastasis site in the liver (magnification, ×40). Lower panel—representative WWOX, Pancytokeratin, pERK, pSTAT3, and amylase immunochemical staining of metastasis site in the lung of KWC mice (magnification, ×10). ***P < 0. 0001. Two-tail unpaired t-test. Quantification graphs are presenting the means ± SD.

Back to article page