Fig. 3: AT7519 arrests glioblastoma cells at the G1/S and G2/M phases of the cell cycle.

A, B After U87MG and U251 cells were treated with different concentrations of AT7519 for 48 h, the cell cycle distribution was detected by flow cytometry. C, D Western blot analysis of cell lysates treated with different concentrations of AT7519 for 48 h. Detection of G1/S and G2/M arrest-related proteins.