Fig. 1: Loss of E13 leads to increased expression of p73β and subsequently, induces growth suppression and sensitizes cells to ferroptosis.

A The level of various p73 isoforms and actin transcripts was measured in isogenic control and E13-KO H1299 cells. B The level of p73 and actin proteins was measured in isogenic control, TAp73-KO, ΔNp73-KO and E13-KO H1299 cells mock-treated or treated with camptothecin (CPT) for 18 h. The HA-tagged TAp73β□□□□□□n were used as a reference control for endogenous TAp73β. C Colony formation assay was performed with isogenic control, TAp73-KO, ΔNp73-KO and E13-KO H1299 cells. The relative density was quantified and shown below each image. *p < 0.05 by Student’s t-test. D Scratch assay was performed with isogenic control, TAp73-KO, ΔNp73-KO and E13-KO H1299 cells. The relative % of wound closure was shown below each image. E Isogenic control and E13-KO H1299 cells were transiently transfected with a scrambled siRNA or a siRNA against TAp73 or ΔNp73, followed by RT-PCR to measure the level of TAp73, ΔNp73 and actin transcripts. F Scratch assay was performed with cells treated in (E).