Fig. 4: Loss of E13 promotes ferroptosis.

A Isogenic control, TAp73-KO, ΔNp73-KO and E13-KO H1299 cells were mock-treated or treated with Erastin for 8 h and representative microscopic pictures were taken to show cell morphology. B Cells were treated the same as indicated in (A), followed by CellTiter-Glo viability assay. Each treatment was performed in triplicates and data were presented as mean ± SEM. *p < 0.05 by student t-test. C Cells were treated the same as indicated in (A), followed by RT-PCR analysis to examine the levels of PTGS2, TFRC, LPCAT4 and actin transcripts. D The level of intracellular cystine was measured in isogenic control and E13-KO H1299 cells by Mass Spectrometric analysis. The analysis was performed in triplicates and data were presented as mean ± SEM. *p < 0.05 by student t-test.