Fig. 2: Overexpression of circIFNGR2 promoted proliferation, invasion and migration capacities of CRC cell. | Cell Death & Disease

Fig. 2: Overexpression of circIFNGR2 promoted proliferation, invasion and migration capacities of CRC cell.

From: CircIFNGR2 enhances proliferation and migration of CRC and induces cetuximab resistance by indirectly targeting KRAS via sponging to MiR-30b

Fig. 2

a The transfection efficiency of circIFNGR2 (named as circIFNGR2), circIFNGR2-shRNA1 (named as shRNA1) and circIFNGR2-shRNA3 (named as shRNA3) in HCT8 and CACO2 cells was analyzed by RT-qPCR. b CCK8 assays manifested the positive effect of circIFNGR2 on CRC cells growth c Representative image of colony formation assay in HCT8 cells. d Statistical analysis of the results of cell colony formation assays. e Representative images of transwell chamber assays in HCT8 cells. f Statistical analysis of the number of invasive cells in the transwell chamber assay. g Wound healing assay results showed the migration of HCT8 cells at 3 specific time intervals. h Statistical analysis of wound healing assay results. i Images of the tumor xenograft model. j Hematoxylin and Eosin (H&E) and Ki-67 immunohistochemical analysis on subcutaneous tumors from mouse tumor xenograft models. k Comparison of tumor volume of indicated cells. l Comparison of tumor weight of indicated cells. m Statistical analysis of Ki-67 positive cells. The error bars represent the means ± SDs from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.005. n Images of the mice spleen-liver metastasis model captured by MRI. o Images of spleen-liver metastasis tumor (left) and corresponding HE staining (right). p Statistical analysis of the number of the spleen-liver metastasis tumors. q Images of tail vein-lung metastasis tumor (left) and corresponding HE staining (right). r Statistical analysis of the number of the tail vein-lung metastasis tumors.

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