Fig. 6: Overexpression of circIFNGR2 lowers the sensitivity of CRC cells to cetuximab. | Cell Death & Disease

Fig. 6: Overexpression of circIFNGR2 lowers the sensitivity of CRC cells to cetuximab.

From: CircIFNGR2 enhances proliferation and migration of CRC and induces cetuximab resistance by indirectly targeting KRAS via sponging to MiR-30b

Fig. 6

HCT8 and CACO2 cells were divided into four groups, which were respectively treated with vector + DMSO, vector + Cetuximab, circIFNGR2 + DMSO or circIFNGR2 + Cetuximab. a CCK8 assay revealed the cell growth ability of the indicated cells. b The transwell chamber assay detected the migration ability of the indicated cells. c Plate colony formation assay confirmed the proliferation ability of the indicated cells. Representative images(left); Statistical analysis of experimental results(right). d Statistical analysis of wound healing assay results for specified cells. e Flow cytometry for apoptosis detection of indicated cells (left). Statistical analysis of flow cytometry results (right). f The image of the tumor xenograft model (n = 5). g Statistical analysis of tumor volume at a specific time point for the four designated groups. h Statistical analysis of tumor weight among the four designated groups. i Immunohistochemical analysis and HE staining of xenograft tumors. The error bars represent the means ± SDs from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.005.

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