Fig. 2: Survivin expression, localization, and phosphorylation in AAs and EAs with TNBC. | Cell Death & Disease

Fig. 2: Survivin expression, localization, and phosphorylation in AAs and EAs with TNBC.

From: PLK1 and AURKB phosphorylate survivin differentially to affect proliferation in racially distinct triple-negative breast cancer

Fig. 2

A BIRC5 expression level in AA and EA patients with TNBC (TCGA dataset). B Relative BIRC5 expression in AA and EA TNBC cell lines from Neve et al. (2006) (B) and our in-house TNBC cell lines (C) (n = 3 each). D Immunoblot showing survivin levels in AA (n = 3) and EA (n = 3) TNBC cell lines. E–G Representative IHC images (E) and bar graphs (F, G) showing survivin H scores in AA and EA patients with TNBC in the Dekalb (F) and Emory (G) cohorts. H IF images showing the localization of survivin (green) in AA and EA TNBC cell lines. Nuclei were stained with Hoechst (blue) and tubulin (red). I Immunoblot showing p-survivin (S20, T117) levels in AA and EA TNBC cell lines (n = 3 each). FPKM fragments per kilobase of transcript per million mapped reads, TPM transcripts per million mapped reads. Bars indicate mean ± SEM. Unpaired two-tailed Student’s t-test with Welch’s correction was used to determine statistical significance (*P < 0.05, ***P < 0.0005, ns = non-significant). Scale bars in E and H are 100 µm and 10 µm, respectively.

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