Fig. 4: Survivin is crucial for cell proliferation and cell cycle progression in AA TNBC cells. | Cell Death & Disease

Fig. 4: Survivin is crucial for cell proliferation and cell cycle progression in AA TNBC cells.

From: PLK1 and AURKB phosphorylate survivin differentially to affect proliferation in racially distinct triple-negative breast cancer

Fig. 4

A–C Representative immunofluorescence images (A, B) and quantification bar graphs (C) showing BrdU (green) incorporation in various AA (A) and EA (B) TNBC cell lines transfected with scrambled or survivin siRNAs. Nuclei were counterstained with Hoechst (blue) and tubulin (red). D–F Bar graphs showing BrdU incorporation in AA and EA TNBC cells treated with survivin siRNA (D), volasertib (E), and barasertib-HQPA (F). Absorbance was measured at 450–540 nm. G–I Flow cytometry analysis depicting various cell cycle phases in AA (G) and EA (H) TNBC cells treated with control (red), survivin siRNA (light blue), YM155 (dark green), volasertib (orange), and barasertib (bright green) and their quantification (I). Data were analyzed using FlowoJo. Bars indicate mean ± SEM. Unpaired two-tailed Student’s t-test with Welch’s correction was used to determine statistical significance (*P < 0.05, ***P < 0.0005, ns = non-significant). The scale bar represents 10 µm.

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