Fig. 7: CPC complex formation is highest in S20-T117 double phospho-mimic survivin mutants. | Cell Death & Disease

Fig. 7: CPC complex formation is highest in S20-T117 double phospho-mimic survivin mutants.

From: PLK1 and AURKB phosphorylate survivin differentially to affect proliferation in racially distinct triple-negative breast cancer

Fig. 7

A Schematic representation of survivin-mutant plasmids. B–D Immunoblots (B, C) showing the levels of CPC proteins in input (B) and IP-bound (C) samples from cells expressing various survivin-WT and mutant plasmids, and their respective quantification (D). E Bar graphs showing the percentage of cell proliferation in control cells and in cells expressing survivin-WT and mutant plasmids. F Schematic illustration of YM155 treatment schedule in mice bearing tumors and surgically implanted with osmotic pumps. G–J Representative tumor images (G), changes in tumor volume (H), and changes in tumor size (I, J) in mice bearing AA (n = 12) and EA (n = 12) TNBC xenografts. Bars represent mean ± SEM. Unpaired two-tailed Student’s t-test with Welch’s correction was used to determine statistical significance (*P < 0.05, **P < 0.005, ns = non-significant).

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