Table 1 List of reagents and antibodies.

1

Lipofectamine RNAiMAX

2%

13,778,075

ThermoFisher Scientific

2

Signal silence Survivin siRNA

100 nM final concentration

6351

Cell Signaling Technology

3

Anti-survivin antibody

1:1000 (WB) 1:100 (IHC)

2808S

Cell Signaling Technology

4

RNAeasy mini kit

74,104

Qiagen

5

iScript cDNA synthesis kit

1×

1,708,891

BioRad

6

SsoAdvanced Universal SYBR Green Supermix

1×

1,725,271

BioRad

7

Protease inhibitor cocktail

1×

P8340

Sigma Aldrich

8

Anti-AURKB antibody

1:500

NBP261493

Novus Biologicals

9

Anti-p-survivin (S20) antibody

1:1000 (WB) 1:100 (IHC-P)

NB11092717

Novus Biologicals

10

Anti-p-survivin (T117) antibody

1:1000 (WB) 1:100 (IHC-P)

MBS003339

MyBioSource

11

Anti-β-actin antibody

1:1000 (WB)

SC-47778

Santa Cruz Biotechnology

12

Goat anti-mouse HRP antibody

1:8000 or 1:10,000

SC-2005

Santa Cruz Biotechnology

13

Goat anti-rabbit HRP antibody

1:8000 or 1:10,000

4050-05

Southern Biotech

14

ECL kit

32106

Thermo Fisher Scientific

15

BrdU cell proliferation kit

2750

EMD Millipore

16

BrdU antibody

1:1000

ab152095

Abcam

17

α tubulin antibody

1:400 (IF)

T9026

Sigma Aldrich

18

Volasertib

15 mg/kg

HY-12137

MedChem Express

19

Barasertib-HQPA

HY-10126

MedChem Express

20

1× phosphate buffer saline

MT21040CV

Corning

21

Propidium iodide

50 µg/mL

P4170-10MG

MilliporeSigma

22

RNase

100 μg/mL

EN0531

Thermo Fisher Scientific

23

37% paraformaldehyde

252549-500 ML

MilliporeSigma

24

Crystal violet

1×

C0775-25G

MilliporeSigma

25

Diva 10× antigen retrieval buffer

1×

DV2004LX

Biocare Medical

26

Anti-Ki-67 antibody

1:100

CRM325C

Biocare Medical

27

pHH3 antibody

1:500

3130

Biocare Medical

28

Anti-PLK1 antibody

1:50

4513 S

Cell Signaling Technology

29

Rabbit HRP antibody

1×

RHRP520L

Biocare Medical

30

Mouse HRP antibody

1×

MHRP520L

Biocare Medical

31

Nude (nu/nu) mice

002019

Jackson Laboratories

32

Barasertib

100 mg/kg

HY-10127

MedChem Express

33

YM-155

10 mg/kg

T2111-SB200

TargetMol

34

ALZET Micro-osmotic Pumps

Model 1004

ALZET

35

Maxi prep kit

12165

Qiagen

36

Lipofectamine LTX Plus

LTX (7.5 μl/well) Plus-reagent (5 μl/2.5 μg plasmid/well) in a 6-well plate

15338030

Thermo Fisher Scientific

37

Anti-Flag-M2 magnetic beads

25 μl/reaction

M8823

MilliporeSigma

38

Anti-Borealin antibody

1:500 (WB)

sc-376635

Santa Cruz Biotechnology

39

Anti-INCENP antibody

1:500 (WB)

ab12183

Abcam

40

Corning BioCoat Matrigel Invasion Chambers with 8.0 µm PET Membrane

354480

Corning

41

PLK1 siRNA,

100 nM final conc

6292, AM5133

Cell Signaling Technology, Thermo Fisher Scientific

42

AURKB siRNA

100 nM final conc

L00332600-0005, AM16708

Horizon Discovery Thermo Fisher Scientific

43

DAB Chromogen Kit

DB801

Biocare Medical

44

PLK1 (NM_005030) Human Untagged Clone

SC110978

OriGene

45

Aurora B (AURKB) (NM_004217) Human Tagged ORF Clone

RC210288

OriGene