Fig. 2: NR modulators inhibited osteosarcoma cell growth by specifically targeting NRs in a dose-dependent manner. | Cell Death & Disease

Fig. 2: NR modulators inhibited osteosarcoma cell growth by specifically targeting NRs in a dose-dependent manner.

From: Nuclear receptor modulators inhibit osteosarcoma cell proliferation and tumour growth by regulating the mTOR signaling pathway

Fig. 2

A–D U2OS, E–H HOS-MNNG, and Saos-2 (I–L) cells were treated with the RARb antagonist, PPARg antagonist, LXR agonist and Rev-Erba agonist at gradient concentrations (1, 5, or 10 μM). The cell confluence was evaluated in real time by the IncuCyte zoom living cell imaging system. n = 6; *P < 0.05, the NR modulator group versus the Ctrl group. M–P Wild-type (Wt) and NR-knockout (KO) U2OS cells were treated with the corresponding NR modulators at a concentration of 10 μM. The cell confluence was evaluated in real time by the IncuCyte zoom living cell imaging system. Mean ± SD, n = 6; *P < 0.05, the NR-KO group versus the Wt group. #P < 0.05, the NR modulator group versus the saline group. △Confluence represents the difference in confluence between the saline group and the NR modulator group. The violin plots show the distribution of the △Confluence of the indicated genotypes. Paired t-tests were used to evaluate the significance. *P < 0.05, the NR KO group versus the Wt group.

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