Fig. 3: Osteosarcoma cell proliferation was repressed by NR modulator treatments. | Cell Death & Disease

Fig. 3: Osteosarcoma cell proliferation was repressed by NR modulator treatments.

From: Nuclear receptor modulators inhibit osteosarcoma cell proliferation and tumour growth by regulating the mTOR signaling pathway

Fig. 3

U2OS, HOS-MNNG and Saos-2 cells were treated with the RARb antagonist, PPARg antagonist, LXR agonist and Rev-Erba agonist at 10 μM for 2 days. A The KI-67 protein levels were evaluated by immunofluorescence. Scale bar: 100 μm. B The relative KI-67-positive area was normalized to the costained DAPI-positive area. Mean ± SD, n = 5; *P < 0.05, the NR modulator group versus the Ctrl group. C Cell lysates were collected. The protein levels of PCNA were evaluated by immunoblotting. D An MTT viability assay was performed to analyse the cell viability of each group. Mean ± SD, n = 5; *P < 0.05, the NR modulator group versus the Ctrl group. E Cell cycle analysis was performed after cell collection, fixation and PI staining. The proportions of cells in the G0/G1, S, and M/G2 phases in each group are shown. Mean ± SD, n = 3; *P < 0.05, the NR modulator group versus the Ctrl group.

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