Fig. 4: FRMD3 downregulated the expression of vimentin and focal adhesion related proteins. | Cell Death & Disease

Fig. 4: FRMD3 downregulated the expression of vimentin and focal adhesion related proteins.

From: FRMD3 inhibits the growth and metastasis of breast cancer through the ubiquitination-mediated degradation of vimentin and subsequent impairment of focal adhesion

Fig. 4

a Western blot analysis of EMT-related proteins in the indicated cells. b GO enrichment analysis of biological processes of FRMD3 positively associated genes in breast cancer. c Western blot analysis of the focal adhesion complex proteins in the indicated cells. d Cell adhesion analysis of MCF10 cells with FRMD3 knockdown and MDA-MB-231 cells with FRMD3 overexpression. The number of cells seeded in fibronectin-coated wells was determined by MTT assay. e Representative images of immunofluorescence staining of exogenous Flag-FRMD3 (red) and endogenous vimentin (green) in MDA-MB-231 cells transiently transfected with pc-flag-FRMD3 and photographed with a laser scanning confocal microscope under ×1000 (upper panel) and ×400 (lower panel) magnification. The nucleus was stained with DAPI (blue). Scale bars: 10 μm (upper panel) and 25 μm (lower panel). f Representative images of immunofluorescence staining of vinculin(red) in MDA-MB-231 cells with stable FRMD3 overexpression or control cells. The nucleus was stained with DAPI (blue). Scale bar, 25 μm. g Western blot analysis of proliferation-related proteins and signaling in indicated cells with overexpression or silencing of FRMD3. h Western blot analysis of the indicated proteins in xenografts shown in Fig. 3c and e. N = 3 biologically independent replicates. The student’s t-test was used to estimate the significance of difference between two groups. Data were presented as means ± s.d. **P < 0.01.

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