Fig. 6: FRMD3 inhibited BRCA cell proliferation and soft agar colony formation by downregulation of vimentin. | Cell Death & Disease

Fig. 6: FRMD3 inhibited BRCA cell proliferation and soft agar colony formation by downregulation of vimentin.

From: FRMD3 inhibits the growth and metastasis of breast cancer through the ubiquitination-mediated degradation of vimentin and subsequent impairment of focal adhesion

Fig. 6

a Western blot analysis of the expression of FRMD3 and vimentin in stable FRMD3-overexpressed BRCA cells transfected with pcGST-vimentin plasmids or in stable FRMD3 knockdown MCF10A cells transfected with siRNA targeting vimentin (siVimentin). b–d MTT assay (b), BrdU incorporation assay (c), and plate colony formation assay (d) were performed to assess cell proliferation of the indicated cells. e Soft agar colony formation assay in T47D cells and MDA-MB-231 cells with FRMD3 overexpression, with or without vimentin overexpression. N = 3 biologically independent replicates. The student’s t-test was used to estimate the significance of difference between two groups. Data were presented as means ± s.d. *P < 0.05; **P < 0.01; ***P < 0.001.

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