Fig. 7: FRMD3 inhibited BRCA cell migration, invasion, and adhesion by downregulation of vimentin. | Cell Death & Disease

Fig. 7: FRMD3 inhibited BRCA cell migration, invasion, and adhesion by downregulation of vimentin.

From: FRMD3 inhibits the growth and metastasis of breast cancer through the ubiquitination-mediated degradation of vimentin and subsequent impairment of focal adhesion

Fig. 7

a–c Scratch wound-healing assay (a), Transwell assay (b), and 3D spheroid invasion assay (c) were performed to detect cell migration and invasion in stable FRMD3-overexpressed MDA-MB-231BRCA cells transfected with pcGST-vimentin plasmids or in stable FRMD3 knockdown MCF10A cells transfected with siRNA targeting vimentin (siVimentin). d Cell adhesion analysis of indicated cells. MTT assay was used to quantify the cells adhered to fibronectin-coated wells. e Western blot analysis of the indicated proteins in corresponding cells with vimentin overexpression or knockdown. N = 3 biologically independent replicates. The student’s t-test was used to estimate the significance of difference between two groups. Data were presented as means ± s.d. **P < 0.01; ***P < 0.001.

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