Fig. 8: The ubiquitin-like domain of FRMD3 was indispensable for its anti-tumor effects on BRCA. | Cell Death & Disease

Fig. 8: The ubiquitin-like domain of FRMD3 was indispensable for its anti-tumor effects on BRCA.

From: FRMD3 inhibits the growth and metastasis of breast cancer through the ubiquitination-mediated degradation of vimentin and subsequent impairment of focal adhesion

Fig. 8

a–d MTT assay (a), BrdU assay (b), plate colony formation assay (c), and soft agar colony formation assay (d) were performed to detect cells proliferation of T47D cells and MDA-MB-231 cells with overexpression of FRMD3, FRMD3-Ubdel, or vector control. e, f The wound-healing assay (e), transwell migration and transwell Matrigel invasion assays (f) were conducted to assess migration and invasion of T47D cells and MDA-MB-231 cells overexpressing FRMD3, FRMD3-Ubdel, or vector control. g Representative images of the 3D spheroid invasion assay in T47D cells and MDA-MB-231 cells overexpressing FRMD3, FRMD3-Ubdel, or vector control. h Cells adhesion analysis of T47D and MDA-MB-231 cells overexpressing FRMD3, FRMD3-Ubdel, or vector control. MTT assay was used to quantify the cells adhered to fibronectin-coated wells. N = 3 biologically independent replicates. The student’s t-test was used to estimate the significance of difference between two groups. Data were presented as means ± s.d. ns not significant; **P < 0.01; ***P < 0.001.

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