Fig. 2: circMYO1C promoted the proliferation and migration of PDAC cells. | Cell Death & Disease

Fig. 2: circMYO1C promoted the proliferation and migration of PDAC cells.

From: m6A-modified circRNA MYO1C participates in the tumor immune surveillance of pancreatic ductal adenocarcinoma through m6A/PD-L1 manner

Fig. 2

A RT-qPCR indicated the circMYO1C expression in PDAC cells (PANC-1, Capan-2, BxPC-3, CFPAC-1) normalized to normal cells (HPDE6). GAPDH acted as the internal control. B pcDNA vector (Capan-2 cell line) and short hairpin RNA (shRNA1/2/3) stable transfection (PANC-1 cell line) were performed following RT-qPCR quantitative analysis. C CCK-8 viability assay was performed for PDAC cells’ proliferation using short hairpin RNA (shRNA1/3) or circMYO1C overexpression. D Transwell migration assay was performed for the migrative ability of PDAC cells. Migrated cells were counted using short hairpin RNA (shRNA1/3) or circMYO1C overexpression. E Wound healing assay was performed for the migrated distance of PDAC cells after 48 h using short hairpin RNA (shRNA1/3) or circMYO1C overexpression. F Ethynyl-2-deoxyuridine (EdU) incorporation assay was performed for DNA synthesis using short hairpin RNA (shRNA1/3) or circMYO1C overexpression. EdU-positive cells were counted. *p < 0.05 and **p < 0.01.

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