Fig. 5: Lysine 168 and lysine 175 are the major acetylation sites of KAT8 and mediate lipolysis of CRC cells. | Cell Death & Disease

Fig. 5: Lysine 168 and lysine 175 are the major acetylation sites of KAT8 and mediate lipolysis of CRC cells.

From: KAT8 acetylation-controlled lipolysis affects the invasive and migratory potential of colorectal cancer cells

Fig. 5

A HCT116 cells were transfected with myc-KAT8 and then treated with PA at 0.2 mM for 24 h. Myc-KAT8 was purified and subsequently separated by SDS-PAGE and stained with CBB. The KAT8 band was analyzed by mass spectrometry. B Alignment of MS-characterized putative KAT8 acetylation residues among different species. C HCT116 cells were transfected with myc-KAT8 WT, K168R or K175R mutant plasmids for a Co-IP assay to detect the level of KAT8 acetylation. D HCT116 cells were transfected with the plasmids above combined with Flag-SIRT6 for a Co-IP assay to detect the level of KAT8 acetylation. E-G HCT116 (E), RKO (F) and SW480 (G) cells were transfected with myc-vector, myc-KAT8 WT or myc-KAT8 2KR mutant plasmids, and then cells were lysed to quantify the amount of glycerol inside cells by glycerol colorimetric assay kit. The bar (−) represents the means (n = 3).

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