Fig. 5: PAK2 is associated with centrosomal protein PLK1.
From: PAK2 is essential for chromosome alignment in metaphase I oocytes
A Confocal microscopy shows immunostaining for PAK2 (green), PLK1 (red), and DNA (blue) in mouse oocytes at the GV (germinal vesicle), GVBD (germinal vesicle breakdown), MI (metaphase I), TI (telophase I), and MII (metaphase II) stages (scale bar, 20 μm). B PLK1 protein levels in control and Pak2-KD oocytes. C Oocytes at the MI stage were fixed and incubated with rabbit anti-PAK2 and rabbit anti-PLK1 antibodies, followed by in situ PLA analysis; oocytes injected with PAK2-siRNA were used as negative controls (scale bar, 20 μm). D Co-IP was performed to determine the interaction between PAK2 and PLK1. Oocytes lysates was incubated with IgG and anti-PAK2 antibody, followed by incubation with protein G beads. The blots of IP eluates were probed with anti-PAK2 and anti-PLK1 antibodies, respectively. E Reciprocal Co-IP was performed with IgG and anti-PLK1 antibody. The blots of IP eluates were probed with anti-PLK1 and anti-PAK2 antibodies, respectively.
