Fig. 6: HDAC inhibitors cooperate with 8-CPT-cAMP to induce neuronal differentiation of human glioma stem cells (GSCs). | Cell Death & Disease

Fig. 6: HDAC inhibitors cooperate with 8-CPT-cAMP to induce neuronal differentiation of human glioma stem cells (GSCs).

From: Differential regulation of H3K9/H3K14 acetylation by small molecules drives neuron-fate-induction of glioma cell

Fig. 6

A Morphology alteration of human GSCs (GSC-1 and GSC-11 cells) treated with V, C, M, CM for 48 h. Pictures were taken at ×20 magnification. Scale bars, 50 μm. B Quantification of EdU-positive cells in GSC-1 and GSC-11 treated with V, C, M, CM for 48 h. C Flow cytometry analysis for testing the protein levels of MAP2/TUBB3 in GSC-1 and GSC-11 cells treated with V, C, M, CM for 48 h. D Western blot analysis for testing H3K9/K14ac and CD133 expression in GSC-1 (left) and GSC-11 cells (right) treated with V, C, M, CM for 48 h. E Immunofluorescent analysis for voltage-gated sodium channels (NaV) in GSC-1 and GSC-11 treated with CM for 72 h. Pictures were taken at ×40 magnification. Scale bars, 25 μm. F, G GO analysis after RNA-seq and heatmaps of dysregulated genes for GSC-11cells treated with V and CM for 48 h. Blue: upregulated genes (CM vs V), red: downregulated genes (CM vs V). Red lines indicate adjusted P = 0.05. H Gene enrichment analysis of genes with altered H3K9ac/H3K14ac tags after ChIP-seq in GSC-11 cells treated with V and CM for 48 h. Red trace lines indicate adjusted P = 0.05. All data was compared to vehicle with one-way ANOVA. n.s., not significant; *P < 0.05; **P < 0.01; ****P < 0.0001. Mean ± SD is shown in the bar plot (n = 3 per group). V, Vehicle (1‰ DMSO); C, 0.5 mM 8-CPT-cAMP; M, 1 μM MS275.

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