Fig. 3: SGK2 knockdown facilitates ferroptosis by downregulating GPX4.
From: SGK2 promotes prostate cancer metastasis by inhibiting ferroptosis via upregulating GPX4
A Protein levels of ferroptosis-related genes were examined in SGK2 overexpressed or knockdown PC3 cells by western blotting. B Western blotting analysis of GPX4 expression in PCa tissues without lymph node metastasis(N0) and PCa tissues with lymph node metastasis(N1). C, D Immunohistochemistry (IHC) analysis of GPX4 expression difference and H-scores of GPX4 among PCa tissues without lymph node metastasis(N0) and PCa tissues with lymph node metastasis(N1) and corresponding Lymph Node Metastases tissues. Scale bars, 20 μm. E Western blot assay showed the reversion efficiency of overexpressed GPX4 after SGK2 knockdown in PCa. F, G Cell viability of PCa cells with SGK2 knockdown, with/without GPX4 overexpression, were measured after treating with Era (5 μM), Era (5 μM) and Ferr-1(5 μM) for 24 h by CCK-8 assay. H Concentrations of MDA were measured in the indicated group. I Intracellular ROS of the indicated group was stained by DCFH-DA and determined by flow cytometry. Data are presented as representative images or as the mean ± SD of three independent experiments. *P < 0.05.
