Fig. 2: Ripk3−/−Caspase8−/− mice and Ripk3−/− mice have comparable increases in MAIT cells.
From: RIPK3 controls MAIT cell accumulation during development but not during infection
A–D 6-8 week old C57BL/6 (WT) (n = 3) and Ripk3−/−Caspase8−/− mice (n = 3) were analysed for T cell populations in the liver, lungs and spleen. A Relative abundance of MAIT cells, and B flow plots showing TCRβ+ lymphocytes with MAIT cells gated. MAIT cell abundance is expressed as a percentage relative to TCRβ+ lymphocytes. C Absolute number of MAIT cells and D absolute number of non-MAIT TCRβ+ lymphocytes. Statistical significance is indicated by ns (p > 0.05); * (p ≤ 0.05); ** (p ≤ 0.01); or *** (p ≤ 0.001) as determined by an unpaired two-tailed t-test with Welch’s correction for unequal variance. E, F Increase in MAIT cells and non-MAIT TCRβ+ cells in older Ripk3−/−Caspase8−/− mice. Older (>16 weeks old) C57BL/6 (WT) (n = 3), Ripk3−/− (n = 4) and Ripk3−/−Caspase8−/− mice (n = 4) were analysed for MAIT and non-MAIT TCRβ+ cells in the spleen. (E) Graphs show the percentage and absolute number of MAIT cells and (F) the absolute number of non-MAIT TCRβ+ T cells. Statistical significance is indicated by ns (p > 0.05); * (p ≤ 0.05); ** (p ≤ 0.01); or *** (p ≤ 0.001) as determined by one-way Brown-Forsythe and Welch ANOVA, followed up with an unpaired two-tailed t-test with Welch’s correction for unequal variance.
