Fig. 3: Neither the deficiency of the necroptotic effector MLKL, nor combined deficiency of both MLKL and the apoptotic effector Caspase-8, affect MAIT cell abundance.
From: RIPK3 controls MAIT cell accumulation during development but not during infection
A Flow plots showing MAIT cell abundance in the thymus, spleen and lungs of 6-8 week old WT and Mlkl−/− and Mlkl−/−Casp8−/− mice. MAIT cell abundance is expressed as a percentage relative to TCRβ+ lymphocytes. B Absolute MAIT cell numbers, C relative MAIT cell abundance showing MAIT cell % relative to total thymocytes for the thymus, and MAIT cell % relative to αβ T cells for all other organs (as in A), and D absolute non-MAIT TCRβ+ lymphocytes in each of the indicated organs from WT (black circles), Ripk3−/− (grey circles) and Ripk3−/−Casp8−/− (hollow circles) mice. B-D Bars show the mean ± SEM (n = 6 mice), pooled from four independent experiments. Statistical significance is indicated by ns (p > 0.05); * (p ≤ 0.05); ** (p ≤ 0.01); or *** (p ≤ 0.001) as determined by one-way Brown-Forsythe and Welch ANOVA, followed up with an unpaired two-tailed t-test with Welch’s correction for unequal variance.
