Fig. 1: NLRP3 inflammasome and GSDMD are strongly expressed and activated in CNS microglia during EAE. | Cell Death & Disease

Fig. 1: NLRP3 inflammasome and GSDMD are strongly expressed and activated in CNS microglia during EAE.

From: IRAK-M suppresses the activation of microglial NLRP3 inflammasome and GSDMD-mediated pyroptosis through inhibiting IRAK1 phosphorylation during experimental autoimmune encephalomyelitis

Fig. 1

A Immunoblot analysis of NLRP3, ASC, full-length (FL) and cleaved caspase-1, IL-1β, and GSDMD in the lumbosacral spinal cords of EAE-induced WT mice at peak disease or CFA-treated mice on day 18 after treatment (n = 10 per group). B Immunohistochemistry showing infiltration of NLRP3, ASC, caspase-1, IL-1β, and GSDMD-positive immune cells in the spinal cords of EAE-induced WT mice at peak disease or CFA-treated WT mice on day 18 after treatment. Scale bar: 50 µm. n = 10 per group. C Immunofluorescent labeling of Iba-1 (green), NLRP3, ASC, caspase-1, or GSDMD (red), and DAPI (blue) demonstrates the expression and process of NLRP3 inflammasome and GSDMD-mediated pyroptosis in the microglia of WT EAE mice at peak disease or CFA-treated WT mice on day 18 after treatment (n = 10 per group). Scale bar: 20 µm. Data are from three representative independent experiments and were analyzed by an unpaired t-test or the Mann–Whitney U test. Error bars show the mean ± SEM. ***P < 0.001.

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