Fig. 4: IRAK-M deficiency promotes activation of microglia, NLRP3 inflammasomes, and GSDMD-mediated pyroptosis in the microglia of EAE. | Cell Death & Disease

Fig. 4: IRAK-M deficiency promotes activation of microglia, NLRP3 inflammasomes, and GSDMD-mediated pyroptosis in the microglia of EAE.

From: IRAK-M suppresses the activation of microglial NLRP3 inflammasome and GSDMD-mediated pyroptosis through inhibiting IRAK1 phosphorylation during experimental autoimmune encephalomyelitis

Fig. 4

A Western blotting of full-length (FL) and cleaved caspase-1, IL-1β, and GSDMD. The corresponding charts show the quantification of target protein expression (n = 4 per group). B Immunohistochemistry for the detection of Iba-1+ microglia infiltrated in the lumbosacral spinal cord at peak disease. The left panel shows representative images. Scale bar: 50 μm. The right panel shows the quantification of cell numbers, respectively. Three sections per mouse were evaluated (n = 4 per group). Data were analyzed by an unpaired t-test or the Mann–Whitney U test and are shown as the mean ± SEM. **P < 0.01, ***P < 0.001.

Back to article page