Fig. 7: IRAK1/TRAF6 combination triggers NLRP3 inflammasome-dependent activities and GSDMD-mediated pyroptosis in EAE. | Cell Death & Disease

Fig. 7: IRAK1/TRAF6 combination triggers NLRP3 inflammasome-dependent activities and GSDMD-mediated pyroptosis in EAE.

From: IRAK-M suppresses the activation of microglial NLRP3 inflammasome and GSDMD-mediated pyroptosis through inhibiting IRAK1 phosphorylation during experimental autoimmune encephalomyelitis

Fig. 7

A Western blot was performed to detect the expression of pIRAK1 and IRAK1 in the lumbosacral spinal cord from all EAE groups at peak disease. B Co-immunoprecipitation was performed by precipitating with anti-IRAK1, followed by western blotting for IRAK1 and TRAF6. One-way ANOVA followed by Tukey’s post hoc analysis was used for RT-PCR and western blot quantification analyses. C The quantification of protein expression pIRAK1/IRAK1(n = 5 per group). D The immunoprecipitation quantification of IRAK1/TRAF6 combination (n = 5 per group). Data are shown as the mean ± SEM. *P < 0.05.

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