Fig. 2: The replacement growth pattern and vascular mimicry (VM) in CRLM. | Cell Death & Disease

Fig. 2: The replacement growth pattern and vascular mimicry (VM) in CRLM.

From: Dynamic differentiation of F4/80+ tumor-associated macrophage and its role in tumor vascularization in a syngeneic mouse model of colorectal liver metastasis

Fig. 2

Immunohistochemistry (IHC) with anti-GFP or anti-S100A4 antibodies tracing of Mc38 cells in varied stages showed a replacement growth pattern of CRLM (A) and positive staining quantification (B). C H&E staining showed remaining hepatic tissues and retained red blood cells within the liver metastases (the red arrow pointed to red blood cells and black to vessel co-option). D GOMORI staining showed the tumor–liver interface was smoothly interacted indicating a replacement growth pattern of the liver metastases. E Serial IHC sections on the respective double staining with anti-CD34, -CD31, -S100A4, or -GFP antibodies, and Periodic acid-Schiff (PAS) showed CD34− PAS+ or CD31−PAS+ vasculogenic mimicry (VM) tubes (red arrows) and S100A4+ PAS+ VM (black arrows) within CRLMs.

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