Fig. 3: F4/80+ macrophages were highly linked to CRLM-adopted liver vessel co-option and VM tubes on tumor vascularization. | Cell Death & Disease

Fig. 3: F4/80+ macrophages were highly linked to CRLM-adopted liver vessel co-option and VM tubes on tumor vascularization.

From: Dynamic differentiation of F4/80+ tumor-associated macrophage and its role in tumor vascularization in a syngeneic mouse model of colorectal liver metastasis

Fig. 3

A IHC with an anti-CD34 antibody staining on serial tissue sections of CRLM at 14 dpi displayed 3-D versions of the CD34+ tumor mass grown along an innate liver vessel (the elliptical white space). B IHC staining on S100A4+ cells showing the tumor cells formed tubular structures in a VM manner during disease progression; the arrow showed circular staining with 14 dpi CRLM. C Representative transmission electron microscopy showed endothelium-lining vessels, tumor cell-lined tubular structure as VM; and macrophages in proximity to VM tubes. *The arrowhead showed the flatted endothelial cell (left) or a round tumor cell (middle); the red arrow showed a few lysosomes within a macrophage (right). D IHC staining on the liver metastases showed tubular expression patterns on respective macrophage-related markers such as Lyve1, S100A9, and F4/80; F4/80+ expression showed proximity to PAS+ channels.

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