Fig. 6: DPT-mediated tumor suppression in BC cells is dependent on YAP.
From: DNMT3a-dermatopontin axis suppresses breast cancer malignancy via inactivating YAP
a CCK-8 assays were conducted to evaluate the effects of DPT and YAP co-transfection on the proliferation of MDA-MB-231 and BT549 cells. b Colony formation assays were performed in either DPT-OE or YAP1-OE BT549 and MDA-MB-231 cells. Column diagrams (lower panel) showed colony numbers of each group. c Left panel: transwell migration assays to evaluate the effects of YAP overexpression on DPT-overexpressed BC cells were shown; right panel: statistical analysis of left panel. d Left panel: transwell invasion assays conducted in DPT/YAP-rescued cells were shown; right panel: statistical analysis of left panel. e A schematic model of the proposed molecular mechanisms show that DNMT3a/DPT/YAP signaling inhibits tumor malignancy in BC. DNMT3a-mediated promoter hypermethylation lead to DPT downregulation. DPT directly interacts with YAP and thus suppresses YAP translocating from cytoplasm to the nucleus. Finally, it inhibits downstream transcriptional activation and impedes tumor development. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
