Fig. 1: Single-cell atlas of E11.5–13.5 mouse ovaries. | Cell Death & Disease

Fig. 1: Single-cell atlas of E11.5–13.5 mouse ovaries.

From: Single cell epigenomic and transcriptomic analysis uncovers potential transcription factors regulating mitotic/meiotic switch

Fig. 1

A E11.5–13.5 female mouse genital ridge sections were stained as indicated. Enlarged images are shown on the bottom; Red shows TRA98, green shows STRA8, and blue shows nuclei; bar = 100 μm. B Identification of the time of meiotic initiation according to chromosome cytospreads; Green shows SYCP3 and blue shows nuclei; bar = 1 μm. C Workflow employed to investigate the molecular mechanism of meiotic initiation by single-cell multiomics. GRs gonadal ridges. D Example of normal and large nuclei (up). Percent of germ cells with a large nucleus and of germ cells identified in the gonadal cell populations using scRNA-seq; bar = 10 μm. E Violin plot of marker gene expression across seven ovarian cell types, including germ cells, epithelial cells, endothelial cells, pregranulosa cells, interstitial cells, immune-related cells, and blood-related cells. F Chromatin accessibility of key marker genes in the different clusters. G, H Single-cell RNA profile (G) and single-cell ATAC profile (H) of ovarian cells of E11.5–13.5 female mouse genital ridge; The barplot on the right shows the number of cells in different cell types.

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