Fig. 3: Identification of female germ cell subclusters.

A, B Single-cell RNA (A) and single-cell ATAC (B) profiles of germ cells; The barplot on the right shows the number of cells in different cell subtypes; The solid black lines represent developmental paths and the arrows represent developmental directions; mitotic mitotic subtypes, trans transition-phase subtypes, prel preleptotene subtypes, lep leptotene subtypes, zyg zygotene subtype. C Number of markers in scATAC-seq and scRNA-seq in the five germ cell subtypes. D Pseudo-time of germ cells in the transcriptome, the root node indicates a development starting point and branch nodes indicate a development direction. E Stra8 expression in pseudo-time. The solid black lines represent developmental paths, and the size of the number represents the order of occurrence. F, G The heatmap (F) of the genes in pseudo-time level and gene ontology annotation (G) of upregulation genes in pseudo-time, respectively; Right shows the upregulation genes. H Pseudo-time of germ cells in chromatin accessibility, the root node represents the starting point of development and the leaf node indicates the development of fate. I Expression of peak related to Stra8 in pseudo-time. J, K The heatmap of the peaks in pseudo-time level and gene ontology annotation of upregulation peaks related genes in pseudo-time, respectively; Right shows the upregulation peaks related genes.