Fig. 3: Analysis of LNROP and OCT2 expression in RAMOS cells after LNROP overexpression using CRISPRa or ectopic copy transduction. | Cell Death & Disease

Fig. 3: Analysis of LNROP and OCT2 expression in RAMOS cells after LNROP overexpression using CRISPRa or ectopic copy transduction.

From: The MYC-regulated lncRNA LNROP (ENSG00000254887) enables MYC-driven cell proliferation by controlling the expression of OCT2

Fig. 3

A RT-qPCR analysis of LNROP and OCT2 expression in RAMOS cells after CRISPR activation using a non-targeting guide (NT) or a targeting sgRNA directed against the TSS of LNROP. Data are mean ± SD of three replicates. B RT-qPCR analysis of LNROP expression in RAMOS cells that are wild type (WT) or transduced with a doxycycline-inducible ectopic copy of LNROP. Expression of LNROP was measured 48 hours after doxycycline addition. Data are mean ± SD of three replicates. C RT-qPCR analysis of OCT2 expression in RAMOS cells transduced with a doxycycline-inducible ectopic copy of GFP (control) or LNROP. Expression of OCT2 was measured 48 hours after doxycycline addition. Data are mean ± SD of three replicates. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant, unpaired t test.

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