Fig. 2: Fpn1 knockout in ECs decreases brain iron accumulation and apoptosis associated with the acute stage of ischemic stroke.

Representative western blot images of the levels of FtL, TfR1, FtH and FPN1 (A) in the contralateral (Con) and ipsilateral (Ips) cerebral cortex on Day 1 after dMCAO. Quantification of the western blot signals of FtL (B), TfR1 (C), FtH (D), and FPN1 (F). E Representative western blot images of the levels of Bcl2, Bax, and p-Erk1/2 in the contralateral (Con) and ipsilateral (Ips) cerebral cortex at Day 1 after dMCAO. Quantification of the western blot signals of Bcl2/Bax ratio (G), and p-Erk1/2 (H). β-actin or GAPDH was used as an internal control. The data are presented as the mean ± SD. n = 6, two-way ANOVA, Tukey’s post hoc comparisons, *p < 0.05, **p < 0.01, ***p < 0.001.