Fig. 1: TRPM7 knockout impairs glucose catabolism.
From: The TRPM7 channel reprograms cellular glycolysis to drive tumorigenesis and angiogenesis
A Gene set enrichment analysis (GSEA) of Hallmark gene sets in TRPM7KO versus WT T24 cells. B Glucose uptake analysis by 2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-glucose (2NBDG). C–E Glycolysis was measured by Extracellular acidification rates (ECAR) in WT and TRPM7KO cells under basal conditions and in response to 10 mM glucose, 3 μM oligomycin or 100 mM 2-deoxy-D-glucose (2-DG). F Relative intracellular lactate in WT or TRPM7KO cells. G Glycolytic intermediates and metabolites quantified by mass spectrometry. Two groups of samples were compared by unpaired two-tailed Student’s t-test.
