Fig. 4: CAR T killing of HeLa target cells is mediated by granzymes, TNFα, and IFNγ.
From: Quantifying requirements for mitochondrial apoptosis in CAR T killing of cancer cells
A, B Annexin V/Hoechst viability staining of HeLa-19 and HeLa-DKO-19 cells following 24 h (A, HeLa N = 5, DKO N = 2) or 48 h (B, HeLa N = 2, DKO N = 1) of treatment with the indicated death ligand or cytokine. C, D Results of Fas, TRAIL, and TNFα blockade in CAR T co-culture experiments. For C N = 3, for D Control N = 3, Anti-TNFα N = 2, Anti-IFNγ N = 2, Anti-TNFα + IFNγ N = 1. The estimated E:T50 values and 95% confidence intervals are presented in E. In F, target cells were co-cultured with CD19 CAR T cells pre-treated with 3,4 dichloroisocoumarin (DCI), N = 6. Viability was assessed by Annexin V / Hoechst staining at 24 h. Each point is a biological replicate, significance corresponds to unpaired t test, **p = 0.0066, ***p = 0.0008, ****p < 0.0001.
