Fig. 6: Loss of Bak and Bax confers resistance to CAR T killing in solid tumor target cells.
From: Quantifying requirements for mitochondrial apoptosis in CAR T killing of cancer cells
A EGFR expression quantified by flow cytometry for paired WT and DKO HCT-116 and HeLa cell lines. Nalm6 included as negative control, Iso = isotype control. Panels B–E depict target cell viability following exposure to EGFR CAR T cells assessed via annexin V/Hoechst staining at 20 h. B, D Pooled data from biological replicates using a single donor’s CAR T cells, with a curve fit using non-linear regression. In C and E, curves were fit for each biological replicate performed, and E:T 50 values are presented for each wildtype/DKO pair (total of 3 donors and 5 replicates). Each point is a biological replicate, significance corresponds to either an unpaired (B, D) or paired (C, E) t test. *p = 0.0156, ***p = 0.0008, ****p < 0.0001.
