Fig. 7: Loss of Bak and Bax confers minimal resistance to CAR T killing in lymphoma target cells.
From: Quantifying requirements for mitochondrial apoptosis in CAR T killing of cancer cells
A Sensitivity of JeKo-1 variants to co-culture with CD19 CAR T cells (Vector N = 5, Bcl-2 N = 5, Bcl-XL N = 3) assessed by annexin V/Hoechst staining at 20 h. B Viability of JeKo-1 variants following 24 h of TRAIL treatment (N = 2). C Sensitivity of Nalm-6 variants to co-culture with CD19 BBz CAR T cells (N = 3, single donor) assessed by annexin V / Hoechst staining at 20 h. D Summary statistics for the biological replicates shown in C as well as for co-culture with 28z CD19 CAR T cells. E Viability of Nalm-6 variants following 24 hours of TRAIL treatment (N = 2). Each point is a biological replicate, significance corresponds to an unpaired (A, B, D, E) t test. **p = 0.0013 in B, =0.0017 in D, ***p = 0.009, ****p < 0.0001.
