Fig. 1: USP10 deubiquitinates RUNX1, which is highly expressed in GBM and positively correlates with MES features.
From: USP10 deubiquitinates RUNX1 and promotes proneural-to-mesenchymal transition in glioblastoma
A DUB siRNA library screening revealed that siRNA-mediated inhibition of multiple DUB genes decreased RUNX1 protein levels. **p < 0.01. B Four Flag-tagged DUBs (USP4, USP7, USP10, and USP36) were expressed in HEK293T cells, and cell lysates were analyzed by IP with Flag beads followed by IB with antibodies against RUNX1 and Flag. C Increasing amounts of USP10-WT or USP10-C424A were transfected into HEK293T cells and RUNX1 expression was detected by Western blot. D Heatmap showing the gene expression levels of USP10, RUNX1 and markers of PN and MES in different GBM samples (n = 30 cases). E Expression of USP10 in NBTs and PN, CL, and MES subtypes of GBM tissues detected by IHC. F USP10 protein levels in NBTs and PN, CL, and MES subtypes of GBM samples detected by Western blot. G USP10 protein levels in NHA, four GBM cell lines and two primary GBM cells. H RUNX1 protein levels in LN229 and GBM2 cells transfected with plasmids expressing USP10-WT or USP10-C424A.
