Fig. 2: Knockdown of RUNX1 inhibited USP10-induced PMT in GBM.
From: USP10 deubiquitinates RUNX1 and promotes proneural-to-mesenchymal transition in glioblastoma
A Levels of USP10, RUNX1, PN markers (Olig2 and PDGFRα) and MES markers (YKL-40, MET, and COL5A1) in U251 and GBM1 cells expressing control vector/USP10 or shCtrl/shRUNX1. Transwell assays were used to assess cell invasion (B) and migration (C) after indicated treatments. Scale bar: 100 μm. Right panel shows the quantification. D Proliferation of indicated cells. E, F Representative images showing the in vivo bioluminescence of GBM. Photons/s/cm2/steradian was represented by colored scale bars. Each group contains 10 mice. G, H Representative Kaplan–Meier survival curves showing the survival rate of indicated groups of mice. I Representative images of HE staining of intracranial xenografts. **p < 0.01; ***p < 0.001.
