Fig. 2: PRR15 silencing is required for TNBC initiation and progression in vitro and in vivo. | Cell Death & Disease

Fig. 2: PRR15 silencing is required for TNBC initiation and progression in vitro and in vivo.

From: PRR15 deficiency facilitates malignant progression by mediating PI3K/Akt signaling and predicts clinical prognosis in triple-negative rather than non-triple-negative breast cancer

Fig. 2

a Proliferation of MDA-MB-231 with stable knockdown of PRR15 and scramble control monitored by the IncuCyte system. b Migration ability of PRR15-silenced MDA-MB-231 compared to control cells assessed by wound-healing scratch assays. c Effect of PRR15 knockdown on the invasive ability of MDA-MB-231 investigated using the transwell invasion assays. d Comparison of the proliferative ability of MDA-MB-231 stably overexpressing PRR15 with control cells by IncuCyte platform. e Cell motility of MDA-MB-231 with ectopic PRR15 and counterparts determined through wound-healing scratch assays. f Effect of PRR15 overexpression on the invasive ability of MDA-MB-231 examined using the transwell invasion assays. g Cell proliferation of PRR15-silenced MDA-MB-231 with or without PRR15 restoration, as well as that of their control cells assessed using Incucyte technology. h Comparison of the scratch healing rate in control, PRR15-knockdown, and PRR15-restored MDA-MB-231. i Analysis of the invasive potential in the control, PRR15-knockdown, and PRR15-restored MDA-MB-231 using the transwell invasion assay. j–q Representative gross images (j, n), volume (k, o), and weight (l, p) of xenograft tumors formed by PRR15-silenced MDA-MB-231 and CAL51 and their controls subcutaneously implanted into Balb/c nude mice (n = 4–5), along with the weight of tumor-bearing mice (m, q) described in Figs. 2j, n. r Representative bright-field (left panel) and H&E-stained (right panel) images of lungs from Balb/c-nude mice after the intravenous injection of PRR15-silenced MDA-MB-231 and counterparts (n = 7). s–u Number of metastatic nodules (s), lung weight (t), and body weight (u) of mice carrying lung metastatic lesions. Scale bars: b 200 μm, c 100 μm, e 200 μm, f 100 μm, h 200 μm, i 200 μm; j, n, 10 mm, r left 5 mm, right 2 mm. Data are presented as mean ± SEM, and analyzed by unpaired t-test. *P < 0.05, **P < 0.01, ***P < 0.001. H&E hematoxylin and eosin.

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